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Natural medicine chemical identification steps

Posted on February 4, 2021 by admin

Literature research

1. Determine what kind of compound the compound is. 2. Explore new compounds.

Determination of compound purity

1. Melting point determination: Determine the compound based on the melting point. 2. Appearance: If the crystal is transparent, the compound is relatively pure. If it is darker, the crystal is not pure. 3. TLC: When the measurement is a single round spot, the compound is pure. If the surface is not smooth and Phenibut Manufacturers has burrs, the compound is not pure. (Three expansion systems to do it)4. PLC: Check whether the peak is a single peak in high liquid phase.

Determination of physical constants

1. Solid: melting point must be measured, optical rotation must be measured2. Liquid: boiling point must be measured, shading rate must be measured

Molecular weight determinationPhenibut on sale 2>1. MS method for molecular weight determination: the data obtained after inaccurate determination is consistent with the referenced data2. HR-MS method for accurate molecular weight determination: decimal point There are four behind. (For example, in 366.2378 in MASS, 7 can be different, but 2 cannot be different. The difference in DIFF is less than 5)

Molecular formula det Phenibut Suppliers ermination method

1. Elemental analysis (EA ): mainly used for the analysis of new compounds2. Mass spectrometry-HR-MS, isotope abundance method3. Hydrogen spectrum, carbon spectrum4 . Unsaturation calculation: Ω1+N4-(N1-N3)/2 such as: C30H48O3 Ω1+30-(48-0)/275. Determination of molecular skeleton : Kinship correlation, exclusive color reaction, pop characteristics, partial synthesis, chemical degradation

Determination of functional groups

Chemical methodSpectroscopy (infrared is The most important and best)

Spectral analysis

Infrared (feature area, fingerprint area)ultraviolethydrogen spectrumCarbon spectrum

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